In recent years, electron microscopy instrumentation and methods have significantly advanced. This progress has greatly benefited both the physical and life sciences, from cryo-EM, which was recognized with the 2017 Nobel Prize in Chemistry, to emerging methods such as low-energy holography and vortex beams. Both communities have developed novel techniques, but open challenges still remain. This conference will explore the interdisciplinary applications of cutting-edge electron microscopy methods and technologies, with an emphasis on identifying and addressing new challenges, and developing the next generation of instrumentation.